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Published online 17 May 2007
Published in Vadose Zone J 6:306-315 (2007)
DOI: 10.2136/vzj2006.0069
© 2007 Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Horizontal Gene Transfer on Surfaces in Natural Porous Media: Conjugation and Kinetics

A. Massoudieh, A. Mathew, E. Lambertini, K. E. Nelson and T. R. Ginn*

Department of Civil & Environmental Engineering, 1 Shields Ave., Univ. of California, Davis, CA 95616

Figure 1
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FIG. 1. Observed vs. simulated transconjugant concentration in E. coli for donors initial concentration F+0 = 4.1 x 103 cell mL–1, recipient initial concentration F0 = 7.6 x 106 cell mL–1, (lag) time spent in exhausted state ({tau}e) = 10 min, (lag) time spent in transconjugant state ({tau}T) = 34 min, and transconjugation rate kaz = 10–6 min–1 bacterium–1.

 

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FIG. 2. Simulated (a) mobile and (b) immobile concentration profiles for donor (F+), recipient (F), exhausted (E), and transconjugant (T) bacteria.

 

Figure 3
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FIG. 3. Mass balance diagram for a finite volume in x{tau} space; shown is a two-dimensional control volume where one of the dimensions corresponds to a conventional spatial coordinate (x) and the other to exposure time ({tau}). Through each side of this control volume is shown the facewise specific fluxes in the directions of space and exposure time, respectively.

 





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